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. 2020 Jan 29;45(4):809–824. doi: 10.1007/s11064-020-02954-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Consequences of an application of iron-free CuO-NPs and CuO-Fe-NPs on the viability and the copper content of C6 glioma cells. The cells were treated without or with CuO-NPs (a, b) or CuO-Fe-NPs (c, d) dispersed in the concentrations indicated in IB-BSA for up to 5 h at 37 °C before the cellular LDH activity (a, c) and the cellular copper content (b, d) were determined. The data shown represent means ± SD of values obtained in five independent experiments. Asterisks indicate the significance of differences of data compared with those of control cells (incubated in the absence of NPs) (*p < 0.05, **p < 0.01, ***p < 0.001; ANOVA)