Figure 5.

Primary human AML are recognized independent of the SNP for PIP4K2A. (A) Recognition of primary human AML by T-cell clone 100 as tested in IFN-γ ELISA (top panel). PIP4K2A genotypes [N/N (light gray), S/N (dark gray), or S/S (black)] are depicted for HLA-DRB1*03 positive primary AML. EBV-LCL 3087 is derived from the patient from whom T-cell clone 100 has been isolated, and EBV-LCL 3089 is from its stem cell donor. Enzyme activity of AEP has been measured in corresponding AML cell lysates (bottom). n.d., not determined. (B) T-cell recognition of primary human CD34⁺ cells has been tested by IFN-γ ELISA (top panel). PIP4K2A genotypes (N/N, S/N, or S/S) are depicted. Both patients are typed positive HLA-DRB1*03:01, patient JH is in addition typed positive for HLA-A*02:01 and viability of CD34⁺ cells is confirmed by recognition of CMV peptide NLV in HLA-A*02:01. Enzyme activity of AEP in CD34 cell lysates is depicted (bottom). One representative of two independent experiments is shown.