Table 1.
Primer sequences of 22 selected genes used for qRT-PCR.
| S.No | Locus Id | Gene Name | Primer sequence (5′-3′) |
|---|---|---|---|
| 1 |
Os02t0559800-01 chr02:21250083..21251343 |
E3 ubiquitin ligase EL5 |
F: GTGATCGAGATCCCCGAAT R: CCAGAGCCTTCTCAGTGACC |
| 2 |
Os02t0624300-01 chr02:24878777..24879932 |
Similar to Y19 protein |
F: TCCGGTGGATCAACTACCTC R: GTTGCCGAGAAGGTCGTG |
| 3 |
Os08t0482600-01 chr08:23853339..23854314 |
Cupredoxin domain containing protein. |
F: TCTGATCGCAATGCTCCTC R: CGTTAGCCTCGAAGGTTTTG |
| 4 |
Os08t0473900-03 chr08:23341357..23343240 |
Similar to Alpha-amylase isozyme 3D |
F: AGCTTGTGTTTGCTGCTCCT R: CTGGAAGAGGACCTGTGCTT |
| 5 |
Os08t0473900-01 chr08:23341289..23343273 |
Alpha-amylase isozyme 3D precursor (EC 3.2.1.1) (1,4-alpha-D-glucan glucanohydrolase) |
F: GTGAAGATCGGGACGAGGTA R: CCTTCTCCCAGACGCTGTAG |
| 6 |
Os08t0466200-01 chr08:22904756..22908287 |
Similar to KUP-related potassium transporter |
F: CTTCAGCAAGAGGTCCATCC R: GTTGATCAGGTACGCCGTCT |
| 7 |
Os02t0569900-01 chr02:21740304..21741960 |
Cytochrome P450 family protein-1500bp |
F: ACACGTTCGAGTGGAAGCTC R: CAAGCACATTGGCAGACTTG |
| 8 |
Os02t0626400-01 chr02:24985294..24989388 |
Phenylalanine ammonia-lyase (EC 4.3.1.5) |
F: ACCACCTGACACACAAGCTG R: TACGAGCTGCCTTCCAAGAT |
| 9 | Os03t0753100-01 chr03:31048351..31055017 | MADS-box transcription factor, Inflorescence and spikelet development |
F: GATCGACGTAGAGGCAGCTC R: GGAGGCTCACTGGAAAACAC |
| 10 | Os10t0531400-01 chr10:20645931..20646880 | Glutathione S-transferase GST 30 (EC 2.5.1.18) |
F: GAAGCTACTGGGGATGTGGA R: CTCGTACGGCAGTGACTTGA |
| 11 | Os03t0401300-01 chr03:16301279..16306089 | Sucrose synthase 2 (EC 2.4.1.13) (Sucrose-UDP glucosyltransferase 2) |
F: GCTGAAGGACAGGAACAAGC R: CAGCTCAACCAGACCAGTCA |
| 12 | Os01t0348900-01 chr01:13903285..13904626 | SalT gene product (Salt-induced protein) |
F: CTGGAGTCCCAAATGGAAAG R: CGTTCCAGACCTTCCAAAGA |
| 13 | Os01t0314800-01 chr01:11863682..11864412 | Late embryogenesis abundant protein 3 family protein. |
F: GGCGGACGAGAAGAAGGT R: GCCGGTAGTACCCGGTCA |
| 14 | Os03t0815100-01 chr03:34166100..34167521 | Similar to OsNAC6 protein |
F: GATGATGGTGCCCAAGAAAG R: AACAGGCTGCTGTTGTTCCT |
| 15 | Os04t0180400-01 chr04:5484865..5486703 | Similar to Cytochrome P450 99A2 |
F: GCTCCTACCCAAAGCTGATG R: CATTATCCGGGGACAAACAT |
| 16 | Os09t0334500-01 chr09:10128837..10131086 | WRKY transcription factor 74 |
F: ACGGTGTTCGACGTGATCTA R: CGTGTCCGTCTCCGTCTC |
| 17 | Os12t0559200-01 chr12:22854749..22860198 | Lipoxygenase (EC 1.13.11.12) |
F: CTCCATCAAGGAGTGGGTGT R: CTGGAGCTCCTTGTCCATGT |
| 18 | Os08t0498100-01 chr08:24586613..24587908 | Similar to Caffeoyl-CoA O-methyltransferase 2 (EC 2.1.1.104) |
F: ATCGAGGTGGGTGTCTTCAC R: TCTCGTAGCTCTCCCTGTCC |
| 19 | Os02t0626100-01 chr02:24973450..24977287 | Similar to Phenylalanine ammonia-lyase |
F: AGCGAGTGGATCCTCAACTG R: CTGAGGAGCTCGACTTGGA |
| 20 | Os10t0465700-01 chr10:17180759..17183189 | Similar to Beta-amylase PCT-BMYI (EC 3.2.1.2) |
F: GTGGTGGAGGAGATGGAGAA R: CCGAGGGAGATGTACTCGAA |
| 21 | Os08t0448000-01 chr08:21873076..21875497 | Similar to 4 coumarate coA ligase1 |
F: TCCACGTCTACTCCCTCCAC R: GCAGCATCTTGACGGTGTC |
| 22 | Os02t0738200-01 chr02:30805988..30807610 | Zinc finger, RING/FYVE/PHD-type domain containing protein |
F: AGCTGCTCAGGCTTCTCAAC R: GTCTCTTCAGCCCCTTGAAA |
| 23 | OsACT1 Lee et al. (2011) | actin |
F: TGGAAGCTGCGGGTATCCAT R: TACTCAGCCTTGGCAATCsCACA |
Genes underlying yield QTLs qyld2.1 and qyld8.2 are shown in bold.