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. 2020 Jan 28;122(6):885–894. doi: 10.1038/s41416-020-0729-6

Fig. 4. LA reduces furin mRNA and protein levels in breast cancer cell lines.

Fig. 4

Furin silencing inhibits the maturation of IGF-1R and IR-α, or furin silencing promotes the pro-IGF-1R and pro-IR accumulation and decreases the IGF-1R and IR-α expression in breast cancer cell lines. MCF7 and MDA-MB-231 breast cancer cell lines were treated with EtOH 2% (NT) or with LA (1 or 2 mM) for 36, 48 and 72 h. The furin protein expression was detected by western blot analysis after 48 and 72 h of LA treatment (1 or 2 mM) in a MCF7 (ERα+) and b MDA-MB-231 (ERα−) cell lines. The furin mRNA level was assessed by real-time quantitative reverse transcription PCR in c MCF7 (ERα+) and d MDA-MB-231 (ERα−) cell lines treated with LA (1 or 2 mM). Data are normalised against 28S mRNA level used as an endogenous control. The results are expressed relative to the levels in control cells set at one. This analysis was performed three times. Pro-IGF-1R, pro-IR, mature IGF-1R and mature IR-α expressions were detected by western blot analysis in e MCF7 (ERα+) and f MDA-MB-231 (ERα−) transfected with two different furin siRNAs or scramble siRNA for 48 h.