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. 2020 Mar 17;11:1419. doi: 10.1038/s41467-020-15233-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Currents (black) and fluorescence (red) from W355N–N370W^* and wt spHCN^* channels using holding voltages of a −80 mV and b −10 mV. Scale bars: 0.1 s, 5 µA, and 1% ΔF/F. c G(V) and F(V) curves of wt^* and W355N–N370W^* channels. Mean ± SEM. d W355-F216, N370-E356, and R367–D471 interactions hold wt* channels closed^7,21. Downward S4 movement breaks these interactions and exposes N370 to water-filled crevice. e W355N–N370W removes stabilizing interactions in closed state with S4 up and destabilize water-filled crevice in open state with S4 down. See Supplementary Figs. 3–5 and Supplementary Movies 1–2 for more details. Source data are provided as a Source Data file.