Table 3.
Saccharomyces cerevisiae strains used for the construction of YE9.
| Strains | Description/relevant genotypea | Ref. |
|---|---|---|
| D452-2 | Wild type; Matα leu2 his3 ura3 | [7] |
| DY02 | Expressing the heterologous d-xylose pathway; D452-2 ald6::TDH3P-XYL1-TDH3T-PGK1P-XYL2-PGK1Tpho13::TEF1P-XYL3-TEF1T |
|
| YE3 | DY02 int#4::CCW12P-gaaA-CCW12T | |
| YE4 | DY02 int#4::PGK1P-lgd1-PGK1T | |
| YE5 | DY02 int#4::TDH3P-gaaC-TDH3T | |
| YE6 | Expressing the heterologous D-xylose and d-galacturonic acid pathway; DY02 int#4::CCW12P-gaaA-CCW12T-PGK1P-lgd1-PGK1T-TDH3P-gaaC-TDH3T |
|
| YE6 YPR1 | YE6 CCW12P-YPR1 | |
| YE6 gaaD | YE6 int#6::CCW12P-gaaD-CCW12T | |
| YE01 | Expressing the heterologous d-xylose, and l-arabinose pathway; D452-2 ald6::TDH3P-XYL1-TDH3T-PGK1P-XYL2-PGK1Tint#1::TEF1P-XYL3-TEF1Tsor1::FBA1P-LAD1-FBA1T-PGK1P-ALX1-CYC1T |
[8] |
| YE9 | Expressing the heterologous d-xylose, l-arabinose, and d-galacturonic acid pathway;YE6 int#7::FBA1P-lad1-FBA1T-PGK1P-alx1-CYC1T |
a
XYL1, XYL2, and XYL3 are derived from Pichia stipitis; gaaA, gaaC, and gaaD are derived from Aspergillus niger; lgd1 and lad1 are derived from Trichoderma reesei; alx1 is derived from Ambrosiozyma monospora.