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. 2020 Jan 15;122(6):801–811. doi: 10.1038/s41416-019-0726-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Examples of objects manually classified as CTCs by individual users (a) or automatically classified as tdEVs using the open-source ACCEPT software (b) on images obtained from CRPC, MBC, mCRC and NSCLC cancer patients. The red contours around the objects indicate the contours as detected by the ACCEPT image analysis algorithm. All objects were isolated from 7.5 mL of blood using the CellSearch system. Scale bars indicate 6.4 μm.