Figure 2.
Study design. The Department of Public Health of the University of Naples Federico II (A) and Genedin s.r.l. (a spin-off of the Department of Public Health, University of Naples Federico II) (B1) develop the SiRe® NGS panel kit (B2). The kit was distributed to n = 4 different institutions [(C) University La Sapienza—Rome, (D) Istituto Oncologico Europeo—Milan, (E) Istituto Tumori Giovanni Paolo II—Bari, (F) Consiglio Nazionale delle Ricerche—Sassari] to analyze a series of n = 160 colon/lung routine samples (G–L) (n = 40 for each institution) following the local NGS workflow, for DNA extraction (M–P), quantification (Q–T), and sequencing on IonTorrent platforms (U–Z), after an alignment phase on a common set of n = 20 shared samples. Permission to publish the figure was obtained by Genedin s.r.l. (a spin-off of the Department of Public Health, University of Naples Federico II).