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. 2020 Mar 11;14:178. doi: 10.3389/fnins.2020.00178

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Copyright © 2020 Schumann, Kietzer, Ebel and Bär.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic illustration of the bivariate phase rectified signal averaging (PRSA) procedure. Step 1: Onsets of skin conductance fluctuations (SCF) served as anchor points (blue circles). Randomly defined anchor points were used as reference condition (black circles). Step 2: Anchor points were transferred to the pupil diameter signal (PD) with a temporal delay of 2.5 s as the mean cross correlation function of skin conductance and PD changes reached its maximum at this time lag. Step 3: Delay-compensated anchor points were used to extract segments of pupil diameter responses (PDR). Step 4: Segments were phase-rectified and normalized. Step 5: PDR segments were averaged and analyzed.