FIGURE 4.

Western-blot analysis of global H3K4me3, H3K36me3, and H3K27me3 in WT plants in response to exogenous SA treatment. (A) Amounts of H3K4me3, H3K36me3, and H3K27me3 were determined on nuclear protein enriched fractions extracted from WT 10-day-old seedlings during exogenous SA exposure using indicated antibodies. Histone H3 total protein and coomassie staining were used as a loading control. (B) Mean densitometry values ± SEM for H3K4me3, H3K36me3, and H3K27me3 were calculated from at least three independent experiments, normalized to H3 and presented relative to WT. Letters indicate significant differences (Student’s t-test with Benjamini–Hochberg FDR correction, P < 0.05).