Fig. 2.

Schematic figure of the proteoliposome assay with reconstituted aquaporin for functional analysis of H₂O₂ transport using a 96-well format. In order to provide the detection system for H₂O₂ transported via the lipid bilayer, Horseradish peroxidase (HRP) and Amplex Red are encapsulated in the lipid vesicles. The aquaporin mediated transport (orange trace) is more efficient than simple diffusion (blue trace) giving rise to a faster increase in the fluorescent signal as compared to background. Figure adapted from Wang et al 2020.