Fig. 5.

Endothelial cell activation and levels of soluble thrombomodulin during reperfusion.

(A) TNFα in perfusate (p = 0.692), (B) IL-6 in perfusate (p = 0.578), (C) soluble thrombomodulin in perfusate (p = 0.985) (D) VWF staining, (E) percentage VWF positive endothelial cells per HPF. There were no statistically significant differences between the groups at the end of reperfusion (Kruskal-Wallis test). Preservation by 24 h of DHOPE did not induce more injury to endothelial cells compared to shorter preservation times. DHOPE, dual hypothermic oxygenated machine perfusion; HPF, high-power field; IL-6, interleukin 6; TNFα, tumor necrosis factor alpha; SCS, static cold storage; sTM, soluble thrombomodulin; VWF, von Willebrand factor.