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. 2020 Feb 29;23(3):100953. doi: 10.1016/j.isci.2020.100953

Figure 6.

Figure 6

Protrusions Increase Cellular Uptake of Glucose

(A) SRS (left panels) and TPEF (right panels) images from MIA PaCa-2 cells incubated with 2-NBDG for 1 h. The TPEF signal was from the 2-NBDG accumulated in the cells. The upper panels are the control group, whereas the lower panels are collected under the starvation condition. Scale bar, 10 μm.

(B) Quantitation of the 2-NBDG intensity for MIA PaCa-2 cells in normal (n = 5) and starvation conditions (with [n = 6] and without protrusions [n = 7]). The bars indicate means ± SEM.

(C) Representative TPEF images of MIA PaCa-2 cells immunostained with an antibody against GLUT1. Scale bar, 10 μm.

(D) Quantitation of fluorescent intensity from labeled GLUT1 in (C) (n = 6). The bars indicate means ± SEM.

(E) Transmission phase contrast (left panels) and TPEF (right panels) images of MIA PaCa-2 and G3K cells incubated with 2-NBDG for 2 h. Scale bar, 10 μm.

(F) Quantitation of the 2-NBDG intensity for MIA PaCa-2 and G3K cells without (MIA PaCa-2: n = 105; G3K: n = 67) and with gemcitabine treatment (MIA PaCa-2: n = 103; G3K: n = 68). The bars indicate means ± SEM. ∗∗p < 0.01, ∗∗∗p < 0.001.