Fig. 6.
The miR-22 inhibitor does not have apparent toxicity or a proliferative effect in WD-induced obese mice.
C57BL/6 male mice were fed a WD after weaning. When those mice were 7-months old, they received OCA (10 μg/g body weight, daily oral gavage), adenovirus negative control, or the miR-22 inhibitor (1×109 PFU, via tail vein, once a week), or a combination of OCA plus the miR-22 inhibitor for 3 weeks. Age- and sex-matched CD-fed mice without any treatment were used as baseline controls. (A) serum ALT, ALP, and endotoxin (LPS) levels. (B) Representative liver sections of Ki-67 immunohistochemistry staining; Ki-67-positive liver cells were counted in 5 random fields (40X) per liver section. Scale bar indicates 100 μm. Data are shown as mean ± SD (n = 4). One-way ANOVA with Tukey's t-test. ALP, alkaline phosphatase; ALT, alanine aminotransferase; CD, control diet; LPS, lipopolysaccharide; OCA, obeticholic acid; PFUs, plaque-forming units; WD, Western diet.