Table 2.

Expression stability (M value) of candidate reference genes calculated by NormFinder under different abiotic stress treatment of Agave sisalana

Rank	Drought		Rehydration		High temperature (± 60 °C)		Cold (± 4 °C)		NaCl (100, 200, 300, 400) mM		Total
	Gene	Stability value	Gene	Stability value	Gene	Stability value	Gene	Stability value	Gene	Stability value	Gene	Stability value
1	β-Tub 4	0.389	ARF2 β-Tub 4	0.236	GAPDH	0.152	CUL-1	0.190	β-Tub 4	0.166	β-Tub 4	0.381
2	PP2A-1 CYC-A	0.464			CYC-A	0.193	WIN-1	0.232	RP II	0.171	WIN-1	0.441
3			GAPDH	0.320	ACT11	0.205	ARF2	0.353	CYC-A	0.188	CYC-A	0.494
4	CUL-1	0.504	WIN-1 ACT11	0.457	WIN-1	0.274	GAPDH	0.630	ARF2	0.282	ACT11	0.585
5	ARF2	0.524			eIF-4A	0.303	CYC-A	0.720	GAPDH	0.294	PP2A-1	0.619
6	RP II	0.599	PP2A-1	0.509	ARF2	0.315	PP2A-1	0.739	WIN-1	0.316	CUL-1	0.666
7	ACT11	0.610	RP II	0.548	CUL-1	0.335	RP II	0.786	ACT11	0.339	ARF2	0.671
8	GAPDH	0.622	EEF1α	0.592	β-Tub 4	0.343	β-Tub 4	0.810	CUL-1	0.343	eIF-4A	0.787
9	eIF-4A	0.687	CYC-A	0.700	EEF1α	0.525	eIF-4A	0.820	UBE2	0.360	GAPDH	0.822
10	WIN-1	0.893	eIF-4A	0.744	PP2A-1	0.819	UBE2	0.845	eIF-4A	0.381	RP II	0.839
11	EEF1α	0.924	CUL-1	0.770	UBE2	1.148	ACT11	0.953	EEF1α	0.485	EEF1α	0.961
12	UBE2	1.825	UBE2	1.802	RP II	1.199	EEF1α	1.433	PP2A-1	0.501	UBE2	1.254