Table 3.

Expression analysis of 12 candidate reference genes by BestKeeper under abiotic stress condition in Agave sisalana

Rank	Drought			Rehydration			Temperature (± 60 °C)			Cold (± 4 °C)			NaCl (100, 200, 300, 400) mM			Total
	GENE	Std.	CV	GENE	Std.	CV	GENE	Std.	CV	GENE	Std.	CV	GENE	Std.	CV	GENE	Std.	CV
1	β-Tub 4	± 0.25	1.15	GAPDH	± 0.12	0.87	CYC-A	± 0.12	0.49	CUL-1	0.06	0.23	RP II	0.12	0.49	WIN-1	0.79	3.02
2	EEF1α	± 0.32	1.12	PP2A-1	± 0.36	1.47	eIF-4A GAPDH β-Tub 4	± 0.30	1.18	ACT11 WIN-1	0.23	0.84	β-Tub 4 CYC-A	0.19	0.78	ACT11	0.89	3.31
3	ACT11		1.10	RP II ARF2	± 0.42	1.98			1.84			1.19			0.73	CUL-1	0.95	3.45
4	ARF2	± 0.36	1.51			2.28			1.56	CYC-A	0.46	1.73	ARF2	0.22	1.02	PP2A-1	1.13	4.32
5	GAPDH	± 0.37	1.67	β-Tub 4	± 0.54	2.68	CUL-1	± 0.34	1.24	β-Tub 4	0.58	2.30	CUL-1	0.26	0.96	CYC-A	1.54	6.40
6	PP2A-1	± 0.42	2.53	CUL-1	± 0.61	2.39	ACT11	± 0.40	1.57	RP II	0.73	2.71	GAPDH	0.33	1.82	RP II ARF2	1.70	7.09
7	CYC-A	± 0.58	2.17	eIF-4A	± 0.73	3.29	WIN-1	± 0.46	1.72	PP2A-1 UBE2	0.82 0.84	3.0	UBE2	0.39	1.71
8	CUL-1	± 0.72	3.01	ACT11	± 0.78	2.96	EEF1α ARF2	± 0.60	2.51				WIN-1	0.39	1.46	β-Tub 4	1.76	7.76
9	WIN-1	± 0.72	2.51	EEF1α	± 0.95	4.26			3.01	eIF-4A	0.93	3.22	eIF-4A	0.44	1.61	UBE2	1.82	8.03
10	eIF-4A	± 0.72	2.81	CYC-A	± 1.11	5.21	PP2A-1	± 1.16	4.61	ARF2	1.07	4.21	ACT11	0.53	1.98	eIF-4A	2.10	7.96
11	RP II	± 0.79	2.83	WIN-1	± 1.51	6.05	RP II	± 1.55	6.17	GAPDH	1.99	8.25	EEF1α	0.60	1.99	GAPDH	2.38	13.3
12	UBE2	± 1.18	5.08	UBE2	± 2.85	13.8	UBE2	± 1.88	8.11	EEF1α	3.06	9.27	PP2A-1	0.71	2.65	EEF1α	3.45	12.5