Table 3.
Source of variation | Oxidative stress and enzymatic antioxidant activities | |||||
---|---|---|---|---|---|---|
MDA contents (nmol g−1 FW) | H2O2 contents (mmol g−1 FW) | SOD activity (U mg−1 protein) | CAT activity (U mg−1 protein) | POD activity (U mg−1 protein) | APX activity (U mg−1 protein) | |
Control | ||||||
Control | 7.75ab ± 0.72 | 0.65d ± 0.03 | 0.43f ± 0.04 | 0.85efg ± 0.06 | 4.70bc ± 0.09 | 0.59 cd ± 0.05 |
10 nM PHF | 7.41b ± 0.14 | 0.77d ± 0.06 | 0.54e ± 0.04 | 0.91def ± 0.10 | 3.65c ± 0.41 | 0.67bc ± 0.08 |
40 nM PHF | 8.70ab ± 0.24 | 0.72d ± 0.06 | 0.43f ± 0.05 | 0.69 g ± 0.04 | 3.68c ± 0.55 | 0.49d ± 0.01 |
80 nM PHF | 8.90ab ± 0.38 | 0.82d ± 0.08 | 0.43f ± 0.05 | 1.00cdef ± 0.05 | 3.50c ± 0.19 | 0.47d ± 0.03 |
120 nM PHF | 7.73ab ± 0.57 | 0.86 cd ± 0.03 | 0.46f ± 0.04 | 1.04cde ± 0.06 | 4.91b ± 0.37 | 0.68bc ± 0.05 |
150 mM NaCl | ||||||
Control | 15.9a ± 1.50 | 1.85a ± 0.06 | 0.73d ± 0.04 | 1.09 cd ± 0.01 | 5.70b ± 0.25 | 0.72bc ± 0.04 |
10 nM PHF | 11.3ab ± 1.13 | 1.37b ± 0.10 | 0.79c ± 0.08 | 1.14c ± 0.09 | 4.58bc ± 0.53 | 0.49d ± 0.03 |
40 nM PHF | 9.08ab ± 0.37 | 1.42b ± 0.11 | 1.01a ± 0.04 | 1.57a ± 0.10 | 6.94a ± 0.46 | 1.06a ± 0.08 |
80 nM PHF | 9.65ab ± 0.73 | 1.13bc ± 0.06 | 1.01a ± 0.08 | 1.37b ± 0.06 | 5.78b ± 0.51 | 0.82b ± 0.04 |
120 nM PHF | 12.3ab ± 0.95 | 1.41b ± 0.10 | 0.89b ± 0.07 | 0.81 fg ± 0.04 | 5.15b ± 0.22 | 0.50d ± 0.07 |
Means with different lowercase letter(s) within a column differ significantly at 5% probability level. MDA, malondialdehyde; H2O2, hydrogen peroxide
SOD superoxide dismutase, CAT catalase, POD peroxidise, APX ascorbate peroxidase