Fig. 5.

Suppression of ischemia/reperfusion (I/R)-induced DNA damage response (DDR) and apoptosis with B12. A-D) Representatives of immunostaining of p-H2A.X (a hallmark of DDR) in kidneys of the four groups of mice. E) semi-quantification of positive staining of p-H2A.X. n = 3. F) Western blot and G) densitometric quantitation of p-Chk1. n = 8. H) Western blot of cleaved caspase 3 (c-Cas3, effector of apoptosis) and caspase 3 (Cas3) and I) the ratios of their densitometrically quantitated amounts in kidneys of four groups of mice. n = 4. G) mRNA level of p53 in the kidneys of the four groups of mice. n ≥ 9. longer line: I/R vs. sham; shorter line: I/R + B12 vs. I/R. *p < 0.05, **p < 0.01.