Fig. 7.

Effects of B12 on early stage of reperfusion injury in vitro. Mouse proximal tubular cells (BU.MPT) were treated with hypoxia/reperfusion (H/R) as described in the materials and methods section. A) B12 increased the survival rate of cells treated with H/R. n = 8. B-E) Representative images of fluorescence of ROS probed by DHE. The signal of fluorescence was markedly increased in cells subjected to H/R (D) but was blunted by B12 treatment (0.3 μM) present throughout the entire H/R procedure (E). F–I) mRNA levels of markers of inflammation and fibrosis. n = 8. longer line: H/R vs. control; shorter line: H/R + B12 vs. H/R. *p < 0.05, **p < 0.01.