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. 2020 Jan 11;20:279–291. doi: 10.1016/j.omtn.2019.11.036

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© 2020 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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OIP5-AS1 Interacted with FXR1 and Could Regulate the Expression of FXR1

(A) Pull-down assay confirmed the interaction between OIP5-AS1 and FXR1 by using OIP5-AS1 sense biotin probe and OIP5-AS1 antisense biotin probe. (B) RIP assay showed the enrichment of OIP5-AS1 expression in anti-FXR1 precipitates. (C) The impact of OIP5-AS1 on FXR1 mRNA level was evaluated by qRT-PCR. (D) FXR1 protein level in sh-OIP5-AS1 transfected cells was evaluated by western blot. (E) Expression correlation between OIP5-AS1 and FXR1 in thyroid cancer tissues. **p < 0.01, ***p < 0.001.