Figure 6.

YY1 Bound to the Promoter of β-Catenin

(A) ChIP assay certified the binding of YY1 to CTNNB1 promoter. (B) Transfection efficiency of pcDNA3.1-YY1 and sh-YY1 were determined by qRT-PCR. (B) Luciferase reporter assay was conducted to evaluate the luciferase activity of CTNNB1 promoter in cells transfected with indicated plasmids. (D and E) The effect of YY1 on β-catenin mRNA and protein levels were estimated. (F) The stability of CTNNB1 was measured after treating actD upon YY1 knockdown or overexpression. (G) The effect of YY1 on β-catenin translocation was determined by immunofluorescence (IF) assay. **p < 0.01.