Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Analysis of EC Samples from Three Validation Experiments (Validation 1, 2, and 3)
| Validation 1 | Validation 2 | Validation 3 | |
|---|---|---|---|
| Ct (DP-CAR) | 24.74 ± 0.08 | 24.25 ± 0.01 | 25.19 ± 0.04 |
| Ct (DP-RNaseP) | 24.83 ± 0.04 | 24.56 ± 0.04 | 25.02 ± 0.09 |
| ΔCt | −0.09 | −0.31 | 0.17 |
| SCG-DP-PCR 2−ΔCt method | 1.06 | 1.24 | 0.89 |
Reactions were performed in triplicates. Ct values are represented as mean ± SD. Mean Ct values from qPCR were used for ΔCt calculation.
