Table 4.
Summary of Differences/Influencing Factors of Experimentally Compared Two Strategies ACM and SCG-DP-PCR
| Factors | Absolute Standard Curve Method (ACM) | SCG-DP-PCR |
|---|---|---|
| Experiment | multiple wells (single PCR reaction/well) | single well (duplex PCR reactions) |
| standard curve | no standard curve required | |
| no genomic DNA within PCR reaction | genomic DNA within PCR reactions | |
| no internal control | RNaseP signal as internal control | |
| extensive experimental setup | fast experimental setup | |
| VCN analysis | extensive: linear regression | fast: relative calculation |
| mathematical extrapolation | 2−ΔCt (DP-Car − DP-RNaseP) | |
| Method validation | standard curve validation | extensive efficiency validation |