FIG 4.

Hierarchical clustering distinguishes between disease states. Heat map with the dendrogram resulting from unsupervised hierarchical clustering performed using 335 transcripts (representing 233 genes) that were differentially expressed (at least a 2-fold change, with a P value of <0.05) relative to healthy controls. The values shown are normalized intensities relative to the mean. Red or blue indicates high or low expression, respectively, of the normalized intensities relative to the mean. The heat map displays five distinct clusters, three containing induced genes and two containing repressed genes. Boldfacing indicates genes that were later identified as classifiers for disease states (Tables 4 and 5). A list of the top 40 genes with greatest changes in LD subjects is presented in Table 2, and all dysregulated genes are provided in Table S1 in the supplemental material.