FIG 3.
Endogenous CD4+ and CD8+ T cells also localize to sections in the genital tract containing NR1 T cells. B6 mice received 106 (A to C) WT RFP NR1, (D to F) IFN-γ−/− RFP NR1, or (G to I) IFN-γR−/− RFP NR1 T cells 1 day prior to transcervical infection with 5 × 106 IFU C. trachomatis. At 3 and 5 days postinfection, genital tracts were subdivided into sections visibly containing NR1 T cells (NR1hi) or not containing NR1 T cells (NR1lo) and assessed by flow cytometry for the number of (A, D, and G) NR1 T cells, (B, E, and H) endogenous CD4+ T cells (CD4+ CD8− of CD3+), and (C, F, and I) endogenous CD8+ T cells (CD8+ CD4− of CD3+). Data were pooled from at least two independent experiments performed with five mice each and were analyzed using paired t tests. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.