TABLE 2.
RpoS mutations and associated NAG growth phenotypes
| Strain/isolatea | Alleleb | Change (position)c | NAG phenotype |
|
|---|---|---|---|---|
| Solidd | Liquid (OD600)e | |||
| PAO1 WT | rpoS (wild type) | None | + | 1.3 ± 0.1f |
| PAO rpoS5 | rpoS5 | Frameshift (20) | − | 0.059 ± 0.004 |
| TR02 | rpoS1 | R308T | + | 0.19 ± 0.01f |
| TR11 | rpoS2 | E295* | − | 0.052 ± 0.001 |
| TR12 | rpoS2 | E295* | − | 0.055 ± 0.003 |
| TR13 | rpoS3 | P210L | − | 0.057 ± 0.004 |
| TR16 | rpoS4 | G284S | − | 0.089 ± 0.004 |
| TR22 | rpoS5 | Frameshift (20) | − | 0.062 ± 0.001 |
| TR25 | rpoS6 | Frameshift (198) | − | 0.057 ± 0.003 |
| TR28 | rpoS7 | M1I | − | 0.062 ± 0.012 |
a
Evolved isolates TR02 to TR28 harbor mutations other than those in rpoS.
b
Allele designation according to isolate number.
c
Amino acid change relative to the RpoS protein sequence of strain PAO1. *, stop codon.
d
+, NAG positive; −, no or marginal growth.
e
Data represent means ± standard deviations (n = 3).
f
P value of <0.05, indicating statistically significant difference from all other values.