TABLE 3.
Results of susceptibility testing for MBL-producing E. coli
|
E. coli strain/ plasmida |
MBL | β-Lactams | MIC (μg/ml) |
||||
|---|---|---|---|---|---|---|---|
| Without inhibitor |
+SFC (8 μg/ml) |
+SFC (32 μg/ml) |
+SPC (8 μg/ml) |
+SPC (32 μg/ml) |
|||
| DH5α/pBC-IMP-1 | IMP-1 | MPM | 1 | 0.031 | 0.031 | 0.031 | 0.031 |
| DH5α/pBC-NDM-1 | NDM-1 | MPM | 64 | 2 | ≤0.25 | ≤0.25 | ≤0.25 |
| DH5α/pBC-VIM-2 | VIM-2 | CAZ | 16/32 | 4 | 2 | 1 | 1 |
| DH5α/pBC-TMB-2 | TMB-2 | MPM | 16 | 0.5 | 0.125 | 0.063 | 0.031 |
| BL21(DE3)/pET-SPM-1 | SPM-1 | MPM | 8 | 0.063 | 0.063 | 0.063 | 0.063 |
| BL21(DE3)/pET-DIM-1 | DIM-1 | MPM | 8 | 2 | 2 | 2 | 0.063 |
| BL21(DE3)/pET-SIM-1 | SIM-1 | MPM | 16 | 2 | 1 | 1 | 0.125 |
| BL21(DE3)/pET-KHM-1 | KHM-1 | MPM | 8 | 4 | 2 | 0.125 | 0.063 |
a
The MIC for E. coli BL21(DE3) was determined using LB broth supplemented with 0.5 mM IPTG (isopropyl-β-d-thiogalactopyranoside).