Distribution of key pathways, including nitrification, the use of organic nitrogen compounds, alternative energy metabolisms, and storage compound metabolisms, in the two almost completely reconstructed comammox genomes acquired in this study (names highlighted in red), previously published comammox Nitrospira genomes, and three completely sequenced genomes of canonical Nitrospira (NOB). Blue indicates the presence and gray indicates the absence of the respective pathway. PhaA, acetyl-CoA C-acyltransferase; PhaB, acetoacetyl-CoA reductase; PhaCE, class III poly(R)-hydroxyalkanoic acid synthase subunits C and E; PhaZ, poly(3-hydroxybutyrate) depolymerase.