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. 2020 Mar 17;11(2):e03175-19. doi: 10.1128/mBio.03175-19

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Copyright © 2020 Yang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Phylogenetic analysis of cyanase sequences. (a) Unrooted maximum-likelihood tree highlighting the lineages that contain cyanases from canonical NOB (blue). Note that the Nitrospira lineage also contains the cyanase of the AOA Nitrososphaera gargensis (24). Branches including only cyanases from nonnitrifiers are not labeled. (b) Expanded view showing the placement of the comammox Nitrospira cyanase from LK70 (red) within the Nitrospira/Nitrososphaera cyanase family. In all, 340 amino acid sequence alignment positions and 93 taxa were considered.