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. 2020 Mar 17;11(2):e03250-19. doi: 10.1128/mBio.03250-19

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Copyright © 2020 Szirovicza et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — SDeV replicates in human and reptilian cell lines. (A) A549 (human lung carcinoma), HEK293FT (human embryonic kidney), HeLa (human cervical cancer), and HepG2 (human hepatocellular carcinoma) cells transfected with Δ-fwd (pCAGGS-SDeV-FWD) were stained at 5 days posttransfection for SDAg (anti-SDAg-AF594 [α-SDAg-AF594], left panels, red). Hoechst 33342 was used to visualize the nuclei. The panels on the right show an overlay of the three images. (B) Boid cell lines I/1Ki (kidney), V/1Ki (kidney), V/2Hz (heart), V/1Liv (liver), and V/5Lu (lung) transfected with Δ-fwd (pCAGGS-SDeV-FWD) were stained at 5 days posttransfection for SDAg (α-SDAg-AF594, left panels, red), and Hoechst 33342 was used to visualize the nuclei. The panels on the right show an overlay of the three images. (C) The transfected boid cells from panel B were allowed to grow, passaged three times, and stained for SDAg (α-SDAg-AF488, left panels, green), and Hoechst 33342 was used to visualize the nuclei. The panels on the right show an overlay of the two images. All images were taken at ×400 magnification using a Zeiss Axioplan 2 microscope, and a 30-μm bar is shown in the bottom right corner of each panel.