Figure 2.

circ-AKT1 Was a Bona Fide circRNA

(A) circRNA sequencing analysis displayed the transcription process of circ-AKT1. (B) Quantitative real-time RT-PCR measured relative expression of circ-AKT1 and AKT1 in the RNase R-treated group. GAPDH was the negative control (left); PCR results measured the amplification primer of circ-AKT1 in cDNA and gDNA (right). (C) Relative expression of circ-AKT1 in CC cell lines was tested by quantitative real-time RT-PCR. (D) Subcellular fractionation assay detected transcript abundance of circ-AKT1 in cytoplasm and nucleus of SiHa and CaSki cells. (E) FISH staining confirmed the expression of circ-AKT1 in cytoplasm (scale bars, 10 μm). *p < 0.05, **p < 0.01.