Figure 3.

circ-AKT1 Promoted Cell Proliferation and Invasion in CC

(A) Quantitative real-time RT-PCR detected relative expression of circ-AKT1 in pcDNA3.1/circ-AKT1-transfected SiHa cells and circ-AKT1-siRNA#1-, circ-AKT1-siRNA#2-, or circ-AKT1-siRNA#3-transfected CaSki cells. (B) CCK-8 detected SiHa and CaSki cell viability in differently transfected conditions. (C) Colony formation assay measured colony number of transfected SiHa and CaSki cells. (D) EdU assay detected positive stained cell percent when overexpressing or knocking down circ-AKT1 (scale bars, 100 μm). (E) Transwell invasion assay detected the invasive ability of SiHa and CaSki cells upon circ-AKT1 overexpression and knockdown (scale bars, 60 μm). **p < 0.01.