Figure 4.

AKT1 Was Upregulated in CC Cells and Promoted Proliferation and Invasion

(A and B) Quantitative real-time RT-PCR detected relative expression of AKT1 in CC cell lines and tissues. (C) Spearman’s correlation analysis was used to confirm the correlation between circ-AKT1 and AKT1 expressions in CC samples. (D) Quantitative real-time RT-PCR measured relative expression of AKT1 in si-AKT1#1-, si-AKT1#2-, and si-AKT1#3-transfected CaSki cells. (E) CCK-8 tested cell viability of CaSki cells. (F) EdU assay detected positive stained cell percent (scale bars, 100 μm). (G) Colony formation assay measured colony number in differently transfected groups. (H) Transwell invasion tested the number of invasion SiHa and CaSki cells in response to the transfection of si-AKT1#1 or si-AKT1#2 compared with control (scale bars, 60 μm). *p < 0.05, **p < 0.01.