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. 2020 Jan 16;20:308–322. doi: 10.1016/j.omtn.2020.01.003

Figure 5.

Figure 5

TGF-β-Induced circ-AKT1 Upregulated AKT1 and Promoted EMT in CC

(A) Quantitative real-time RT-PCR detected the expressions of circ-AKT1 and AKT1 in SiHa cells treated with TGF-β at the dose of 0, 1, 2, and 5 ng/mL. (B) Western blot assay displayed the expressions of AKT1, E-cadherin, and N-cadherin in SiHa cells treated with TGF-β at the dose of 0, 1, 2, and 5 ng/mL. (C) Quantitative real-time RT-PCR measured the expression of AKT1 in SiHa and CaSki cells upon circ-AKT1 overexpression and knockdown. (D) IF (immunofluorescence) staining detected the fluorescence intensities of E-cadherin and N-cadherin in SiHa and CaSki cells upon circ-AKT1 overexpression and knockdown (scale bars, 40 μm). (E) Western blot assay tested the expressions of AKT1, E-cadherin, and N-cadherin in SiHa and CaSki cells upon circ-AKT1 overexpression and knockdown. *p < 0.05, **p < 0.01.