Figure 6.

circ-AKT1 Sequestered miR-942-5p to Regulate AKT1 in CC

(A) The Venn diagram showed that miR-942-5p and miR-338-3p were in the intersection of prediction results of PITA and CircInteractome databases. (B) Quantitative real-time RT-PCR detected relative expression of miR-942-5p and miR-338-3p in CC cell lines. (C) Quantitative real-time RT-PCR was used to detect the expression of miR-942-5p upon circ-AKT1 overexpression and knockdown in SiHa and CaSki cells. (D) Quantitative real-time RT-PCR measured the expression of miR-942-5p in CC samples (left). Spearman’s correlation curve showed the correlation of miR-942-5p with circ-AKT1 and AKT1 (right). (E) The binding sites between AKT1 and miR-942-5p, and the binding sites between circ-AKT1 and miR-942-5p were predicted by starBase. (F) Pull-down assay detected the input recovery of circ-AKT1 and miR-942-5p in SiHa and CaSki cells. (G) RIP assays measured the input recovery of circ-AKT1, miR-942-5p, and AKT1 in SiHa and CaSki cells. (H) Luciferase reporter assay was used to examine the luciferase activity of circ-AKT1-WT and circ-AKT1-MUT in transfected 293T cells. **p < 0.01, ***p < 0.001.