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. 2020 Jan 16;20:308–322. doi: 10.1016/j.omtn.2020.01.003

Figure 7.

Figure 7

circ-AKT1 Regulated CC Progression through the miR-942-5p/AKT1 Axis

CaSki cells were transfected with siRNA, circ-AKT1-siRNA#1, circ-AKT1-siRNA#1 + miR-942-5p inhibitor, or circ-AKT1-siRNA#1 + miR-942-5p inhibitor + si-AKT1#1 for subsequent assays. (A) Quantitative real-time RT-PCR detected AKT1 expression in each group. (B) Western blot assay measured the expressions of AKT1, E-cadherin, and N-cadherin in each group. (C) CCK-8 tested cell viability of CC cells in each group. (D) Colony formation assay measured colony number in differently transfected groups. (E) EdU assay detected positive stained cell percent in each group (scale bars, 100 μm). (F) Transwell invasion assay was used to detect the invasive ability of CC cells in each group (scale bars, 60 μm). *p < 0.05, **p < 0.01.