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. 2020 Jan 16;20:308–322. doi: 10.1016/j.omtn.2020.01.003

Figure 8.

Figure 8

circ-AKT1 Facilitated CC Progression In Vivo

Nude mice were injected with SiHa cells transfected with pcDNA3.1 or pcDNA3.1/circ-AKT1. (A) Pictures of xenografts in mice injected with SiHa cells transfected with pcDNA3.1 or pcDNA3.1/circ-AKT1. (B) The growth curve of xenografts in mice injected with SiHa cells transfected with pcDNA3.1 or pcDNA3.1/circ-AKT1. (C) The final tumor volume and weight in mice injected with SiHa cells transfected with pcDNA3.1 or pcDNA3.1/circ-AKT1. (D) Quantitative real-time RT-PCR measured the expressions of circ-AKT1, AKT1, and miR-942-5p in mice injected with SiHa cells transfected with pcDNA3.1 or pcDNA3.1/circ-AKT1. (E) Spearman’s correlation curve showed the correlation between circ-AKT1 and AKT1, and the correlation of miR-942-5p with circ-AKT1 and AKT1 in mice injected with SiHa cells transfected with pcDNA3.1/circ-AKT1. (F) Western blot detected the expressions of AKT1, E-cadherin, and N-cadherin in mice injected with SiHa cells transfected with pcDNA3.1 or pcDNA3.1/circ-AKT1. (G) circ-AKT1 was induced by TGF-β and could sequester miR-942-5p to upregulate AKT1 expression, so as to promote cell proliferation and invasion in CC. *p < 0.05, **p < 0.01.