Skip to main content
. 2019 Oct 22;9(4):569–585. doi: 10.1016/j.jcmgh.2019.10.005

Figure 7.

Figure 7

Butyrate increases RNA Pol II recruitment to the CPT-1A promoter. Primary hepatocytes were left untreated (UT) or treated with 50 mmol/L ethanol for 12 hours (Ethanol). Cells were pretreated with sodium butyrate (NaB, 2 mmol/L) for 30 minutes before ethanol (Ethanol + NaB). ChIP analysis was performed using chromatin fragments immunoprecipitated with anti-RNA Pol II antibody and ChIP real-time quantitative PCR was performed using primer pairs specific for region I on the CPT-1A promoter. Nonimmunoprecipitated chromatin was used as input. Data were analyzed by analysis of variance and represented as means ± SD, n = 4. P value < .05 (a) when compared with UT and (b) when compared with ethanol.