Figure 3.

KLF5 is required for long-term intestinal stem cell survival. (A) Representative immunofluorescence images of EGFP, RFP, KLF5, and DAPI of PSI crypts of Lgr5^Ctrl and Lgr5^ΔKlf5 mice scarified at day 19, 33, or 61. Yellow arrowheads mark RFP⁺ crypts. Magenta arrowheads mark crypts with non-RFP, Lgr5^EGFPhi cells. Scale bar represents 20 μm. (B, C) Quantification of the percentage of (B) RFP⁺ crypts and (C) crypts with non-RFP, Lgr5^EGFPhi cells of Lgr5^Ctrl and Lgr5^ΔKlf5 mice at 5, 12, 19, 33, and 61 days. Data are expressed as mean ± SD, n = 3–6 mice per group. ∗P < .05 by Mann-Whitney U test. (D, E) Representative immunofluorescence images of RFP, KLF5, DAPI, and (D) EdU or (E) TUNEL of PSI crypts of Lgr5^Ctrl and Lgr5^ΔKlf5 mice injected with 3 hours EdU pulse treatment at day 19. Scale bar represents 20 μm.