Table 2.
Number of NoV tests in different regions with comment about main changes in testing methods in regional laboratories, Denmark 2011–2018
| Region | No. of KMAs active during study period | Year | Comment | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 2011 | 2012 | 2013 | 2014 | 2015 | 2016 | 2017 | 2018 | |||
| Northern | 1 | 1102 | 1208 | 985 | 671 | 1173 | 719 | 822 | 1153 |
January 2010 – Norovirus antigen test (EIA Test from Oxoid); July 2013 – Introduction of RT-PCR (Altona Diagnostics); January 2015 – Change to GeneXpert (Xpert Norovirus, Cepheid) |
| Central | 2 | 0 | 0 | 0 | 66 | 522 | 707 | 580 | 954 |
October 2014 – Introduction of in-house RT-PCR; March 2015 – Expanding testing method for whole region |
| Southern | 3 | 3351 | 4068 | 4154 | 4028 | 4680 | 4012 | 4463 | 5854 |
Before 2013 – Use of individual test and package for viral diseases (NoV + rotavirus); After 2013 – Introduction of PCR package for viral diseases (NoV + rotavirus + adenovirus + sapovirus + astrovirus); January 2018 – Introduction of GeneXpert |
| Zealand | 1 | 0 | 115 | 749 | 1539 | 2456 | 2977 | 3716 | 5477 | September 2012 – Introduction of in-house RT-PCR |
| Capital | 4 | 4312 | 3855 | 9130 | 10 264 | 13 556 | 15 417 | 19 150 | 24 241 |
2013 – Introduction of in-house RT-PCR in one laboratory; 2010-2016 – Use of package for viral diseases (NoV + rotavirus + adenovirus) by RT-PCR (BD-max); January 2016 – Change to gastroenteritis package (virus + bacteria) by RT-PCR (C-gene) |
| Reference laboratory | 1 | 6256 | 4494 | 1384 | 974 | 517 | 497 | 401 | 648 | January 2015 – Diagnostic performed mainly due to foodborne outbreaks |
| Total | 12 | 15 021 | 13 740 | 16 402 | 17 542 | 22 904 | 24 329 | 29 132 | 38 327 | |