Fig. 9.

Real time-qPCR evaluation of top 10 differential brain transcripts identified by RNAseq. Expression levels of candidate genes were quantified for Pank1^−/−Syn-Pank2^−/− (dKO) and matched wild-type (WT) brains using RT-qPCR as described in Materials and methods. RNA was isolated from brains of 3 males and 3 females each for dKO and WT mice at P9-P12 or P19-P21. A. Hbb1 and Hbb2 encode proteins in hemoglobin synthesis. B. Hspa1a, Pla2g4a, LCat and Alas genes encode proteins for heat-shock protein 70, an isoform of phospholipase A2, lecithin-cholesterol acyltransferase, and aminolevulinic acid synthase, respectively. C. Matn and Ma3k6 encode matrilin cartilage protein and a mitogen activated protein kinase, respectively. D. Hif3a, Pdk4 and Pipc encode hypoxia inducible factor 3a, pyruvate dehydrogenase kinase 4, and cyclophilin C, respectively. Pipc was included in the analysis as a negative control. The data represents the combined data from 3 males and 3 females, the mean ± SEM of triplicate determinations with independent samples and statistical significance was calculated using non-parametric t-test comparing age matched WT and dKO mice, the values for which are shown in red.