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. 2020 Mar 13;13:2235–2246. doi: 10.2147/OTT.S242939

Figure 2.

Figure 2

MiR-154-5p overexpression inhibits invasion and migration in 5-8F and CNE-2 cells. 5-8F and CNE-2 cells were transfected with miR-154-5p mimic and mimic NC, respectively. (A) Expression of miR-154-5p in 5-8F and CNE-2 cells after transfection. (B) The effect of overexpression of the miR-154-5p on proliferation in 5-8F and CNE-2 cells was detected by CCK-8 assay. (C) Colony formation was performed to evaluate the colony-forming ability of NPC cells. (D and E) Wound healing assay showed migration in 5-8F and CNE-2 cells. Scale bar, 100 μm. (F–H) Transwell assay was used to assess migration and invasion ability of cells transfected with miR-154-5p mimic or mimic NC. Scale bar, 100 μm. Data are reported as mean ± SD of three independent experiments. **P<0.01.