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. 2020 Mar 11;11:298. doi: 10.3389/fphar.2020.00298

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Copyright © 2020 Feng, Wan, Zhang, Yu, Shao, He, Wan and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The protein strip diagram and protein expressions level of PI3K-Akt pathway related proteins in each group. (A) Band diagrams of p-Akt, p-PI3K, Akt, and PI3K in brain tissue of each group of rats determined by western blot. (B) The expression of p-Akt in brain tissue of each group rats determined by western blot. (C) The expression of p-PI3K in brain tissue of each group rats determined by western blot. (D) The expression of Akt in brain tissue of each group rats determined by western blot. (E) The expression of PI3K in brain tissue of each group rats determined by western blot (n = 4). Compared with the sham operation group, ^▲▲p < 0.01; compared with the MCAO group, *p < 0.05, **p < 0.01; compared with the LY294002 group, ^#p < 0.05, ^##p < 0.01. Sham, sham operation group; MCAO, middle cerebral artery occlusion; LY294002, 2-(4-morpholinyl)-8-phenyl-chromone; DHI, Danhong Injection; NMDP, nimodipine.