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. 2020 Mar 13;12:1913–1927. doi: 10.2147/CMAR.S228718

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© 2020 Wong et al.

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Effects of caspases inhibition on VER/DOC- and VER/VCR-induced apoptosis and autophagy. (A) Pre-treatment of pan-caspase inhibitor (Z-VAD, 50 μM) for 1 hr followed by VER/DOC or (B) VER/VCR co-treatment for 48 hrs and analyzed by Annexin V assay of flow cytometry on A549/D16 and A549/V16 cells, respectively. (C and D) Expressions of PARP, cPARP LC3B and LC3B-II were detected by Western blot analysis with the same treatment conditions as (A and B). *p<0.05 was considered statistically significant.