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Above: Structure of the dye Nile Blue and the labeled lipid Rhodamine B-DOPE (1,2-dioleoyl-sn-glycero-3-phospho-ethanolamine-N-lissamine rhodamine B sulfonyl (ammonium salt)). Below: Spectral overlap between Rhodamine B emission and Nile Blue absorption, confirming the possibility of Förster resonance energy transfer (FRET).
