Figure 9.

Effects of COX‐2, EP2 and PKA inhibitors on E‐cadherin internalization in YAP (5SA) cells during cell competition. Representative images of immunofluorescent staining of DAPI (blue; nuclei), E‐cadherin (green) and CMTPX (red; labeled YAP (5SA) cells or YAP (5SA) cells expressing shEP2). Labeled YAP (5SA) cells or labeled YAP (5SA) cells expressing shEP2 were mixed 1:50 with unlabeled normal MDCK cells or unlabeled MDCK cells expressing shEP2, as indicated. At 24 hr postseeding, cocultures were treated for 22 hr with Dox plus NS398 (50 μM), PF‐04418948 (10 μM) or H‐89 (50 μM). “Enlarged,” high magnification images of the inset boxes in the E‐cadherin panels. Results are representative of three trials. Scale bar, 10 μM