Figure 4.
Reactive oxygen species formation in irradiated cultured human fibroblasts after pretreatment with LicA. After incubation with LicA at various concentrations, cells were exposed either to (A) UV (2.5 J/cm2) or (B) VIS >400 nm, 150 J/cm2. An Oriel 1600 W Solar Simulator filtered for UV irradiation and another Oriel 1600 W Solar Simulator filtered for VIS irradiation were used. Gray bars show oxidative stress levels without irradiation, and black bars represent the additional oxidative stress induced by irradiation. Results of unirriadiated and untreated cells were set to 100%; n = 7; mean ± SD. Significant differences were marked (*P < .05, **P < .01, ***P < .001)