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. 2020 Mar 17;8(1):e000316. doi: 10.1136/jitc-2019-000316

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© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Numbers of circulating lymphocytes during treatment. The absolute numbers of white blood cells (A) and lymphocytes (B) were counted by clinical blood tests. The percentages of peripheral blood Vα24+Vβ11+ iNKT cells (C), CD56+CD3− NK cells (D), CD3+CD8+ T cells (E), CD45RA−CCR7+ effector CD8+ T cells (F), CD45RA−CCR7− effector memory CD8+ T cells (G), and PD-1+CD8+ cells (H) during treatment were assessed by flow cytometry analysis, and the absolute numbers of these cells were calculated using full blood counts. The linear mixed effect model for repeated measurements was used to estimate the geometric mean, SE, and 95% CI of the mean of each measuring point of immune monitoring, which can address all available post-baseline data. *p<0.05, **p<0.01, ***p<0.001. NK, natural killer.