Figure 3.
Immunofluorescence staining of TJ proteins in the intestine of rats from different experimental groups. (a) Images of occludin immunostaining, Bar 50 µm; (b) Analysis of occludin expression with immunostaining; (c) Images of ZO‐1 immunostaining, bar 50 µm; (d) Analysis of ZO‐1 expression with immunostaining; (e) Images of claudin immunostaining, bar 10 µm; (f) Analysis of claudin expression with immunostaining; (g) Images of JAM‐A immunostaining, bar 10 µm; (h) Analysis of JAM‐A expression with immunostaining. A.U., arbitrary units. Rats were pretreated by oral gavage with B. subtilis BSB3 (BSB3) or with PBS and were either exposed forced running or remained sedentary (n = 6 rats per condition). BEx, pretreated with BSB3, undergoing forced running; BCont, pretreated with BSB3 remaining sedentary; PEx, pretreated with PBS, undergoing forced running; PCont, pretreated with PBS remaining sedentary. Data with different superscript letters signify groups are significantly different (P < 0·05). [Colour figure can be viewed at https://www.wileyonlinelibrary.com]