Figure 3.

Knockdown or blockage of CD11b reduces sialidase‐induced phagocytosis in BV‐2 cells and primary rat microglia, respectively. (a) Uptake of 5 μm bead into BV‐2 microglia that were pretreated with nontargeting (NT) or CD11b targeting siRNA. Phagocytic uptake was measured in three independent knockdown experiments and data normalized to nontargeting siRNA (si‐NT). Error bars represent SEM. Statistical analysis was performed by one‐way ANOVA with Tukey's post hoc test, *p < .5 versus si‐NT control, ^# p < .5 versus si‐NT sialidase. (b) CD11b expression 48 hr after siRNA mediated knockdown of CD11b. Data was normalized by beta‐actin gene expression and measurements represent three independent knockdown experiments with error bars representing SEM. Statistical analysis was performed with an unpaired t test, **p < .01. (c) Uptake of beads in primary microglia after stimulation with sialidase in the presence or absence of blocking antibody CD11b or isotype control. N = 3 independent phagocytosis assays with error bars representing SEM. Statistical analysis was performed by one‐way ANOVA with Tukey's post hoc test, **p < .01 versus isotype + control treatment, ^## p < .01 versus sialidase + anti‐CD11b treatment. (d) Binding of FITC‐labeled human fibrinogen to vehicle treated or desialylated primary rat microglia. Data presented as mean fluorescence values of three independent binding assays. Statistical analysis was performed by repeat measure (RM) one‐way ANOVA followed by Sidak's post hoc test, *p < .05